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Product info
- Product name:Kovacs Indole Reagents Kit
- Product code:CCSJ-1
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Packaging Specifications
Core Parameters
Description
Kovacs Indole Reagent Kit
Intended Use
For indole test (indole assay) of microorganisms.
Storage Condition & Shelf Life
Store at 2–8°C; shelf life: 12 months.
Kit Components
表格
| Component Name | Alias | Specification | Preparation Formula |
|---|---|---|---|
| Kovacs Indole Reagent | Kovacs Reagent | 5 mL × 2 bottles | Dissolve 5 g of p-dimethylaminobenzaldehyde in 75 mL amyl alcohol, then slowly add 25 mL concentrated hydrochloric acid. |
Operating Procedures
- Inoculate pure bacterial culture into 1 mL tryptophan broth, tryptone water or other suitable medium, incubate at 36 ± 1°C for 24 ± 2 h.
- Add 2–4 drops of the reagent and mix thoroughly.
- Observe the result immediately, or leave at room temperature for 5–10 minutes before observation.
Reaction Principle
Certain bacteria can decompose tryptophan in peptone to produce indole. Indole can be detected via color reaction: indole combines with p-dimethylaminobenzaldehyde to form rosindole, a red-colored compound.
Result Interpretation
- Positive: A distinct red ring appears on the upper layer of the medium.
- Negative: The solution turns yellow, orange or brown without red ring formation.
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