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Product info
- Product name:Voges-Proskauer Reagent
- Product code:CCSJ-3
Packaging Specifications
- Outer Packaging Size:0 × 0 × 0 m
- Gross Weight:0 kg
- Net Weight:0 kg
- Volume:0
Description
Voges-Proskauer (V-P) Test Reagent Kit
Intended Use
For microbial V-P biochemical test.
Storage Condition & Shelf Life
Store at 2–8°C; shelf life: 12 months.
Kit Components
表格
| Component Name | Alias | Specification | Preparation Formula |
|---|---|---|---|
| Solution A | 6% α-Naphthol Ethanol Solution | 5 mL × 3 bottles | Weigh 6.0 g α-naphthol, dissolve with absolute ethanol and dilute to 100 mL. |
| Solution B | 40% Potassium Hydroxide Solution | 5 mL × 1 bottle | Weigh 40 g potassium hydroxide, dissolve with distilled water and dilute to 100 mL. |
Reaction Principle
Some bacteria decompose glucose in glucose peptone water medium to generate pyruvic acid. Pyruvic acid undergoes condensation and decarboxylation to form acetoin. Under strong alkaline conditions, acetoin is oxidized by oxygen in the air to diacetyl. Diacetyl reacts with guanidyl groups in peptone to produce a red compound, which is defined as a positive V-P reaction.
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